Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Hyaluronan synthase 3 promotes plaque inflammation and atheroprogression

doi: 10.1016/j.matbio.2017.09.005

Figure Lengend Snippet: Detection of HAS isoenzymes in human atherosclerotic lesions and association of Has3 expression with early macrophage accumulation in murine lesions. A Representative images of HAS1,-2 and -3 detection via immunohistochemical stainings and Ct values of 18S, HAS1, HAS2, and HAS3v1 in human atherectomy specimen as determined by qPCR; mean ± SEM; n = 6–12. B Left, mRNA expression of Has1, Has2, and Has3 in aortas of Apoe−/− mice at different ages; n = 2–3; means ± SEM. Right, quantification of the area fraction of HA staining and Mac2 in aortic root sections at different ages of Apoe−/− mice; mean ± SEM; n = 3–7. C,D Depiction of HA (C) and Mac2 (D) stainings of aortic roots of 6-, 10-, 14-, and 19-week-old Apoe−/− mice.

Article Snippet: Paraffin embedded sections of atherectomy specimens were stained for the HAS isoenzymes HAS1, HAS2, and HAS3 in consecutive sections using HAS1 (G-17, sc-34021), HAS2 (S-15, sc-34067), HAS3 (E-15, sc-34204) primary antibodies (Santa Cruz, Dallas) and secondary anti-goat antibody (Vector).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Hyaluronan synthase 3 promotes plaque inflammation and atheroprogression

doi: 10.1016/j.matbio.2017.09.005

Figure Lengend Snippet: HAS3 in human and murine T cells. A, mRNA expression of the three hyaluronan synthases, Has1, Has2, and Has3 by activated T cells normalized to 18S expression levels. Data are for pooled CD4+ T cells isolated from 4 mice, activated using anti-CD3/28 beads, and measured in triplicate. B, HAS3 mRNA expression by activated, well characterized TH1 and TH2 clones. The clones were previously described in Bollyky et al., Cellular and Molecular Immunology, 2010. Panel A includes 12 measurements for each gene; 3 mRNA assessments from each of 4 animals. The error bars shown represent the standard error of the mean. Panel B includes a total of 12 measurements; 3 mRNA assessments from a total of 4 human T-cell clones; 2 clones for each condition. The error bars shown represent standard deviation; *p < 0.05. C, CD3+ T-cells were isolated from spleens of Has3−/−/Apoe−/− deficient mice and Apoe−/− mice (8–12 weeks old) and stimulated with IL-1β (10 ng/ml) for 3 h. Subsequently Has3 mRNA was analyzed by qPCR; means ± SEM; n = 6; *p < 0.05.

Article Snippet: Paraffin embedded sections of atherectomy specimens were stained for the HAS isoenzymes HAS1, HAS2, and HAS3 in consecutive sections using HAS1 (G-17, sc-34021), HAS2 (S-15, sc-34067), HAS3 (E-15, sc-34204) primary antibodies (Santa Cruz, Dallas) and secondary anti-goat antibody (Vector).

Techniques: Expressing, Isolation, Clone Assay, Standard Deviation